|
KMID : 0379119920200010044
|
|
Korean Journal of Mycology
1992 Volume.20 No. 1 p.44 ~ p.50
|
|
|
Protoplast Formation and Fusion between Anastomosis Groups of Rhizoctonia solani
|
|
|
|
|
Abstract
|
|
|
|
The protoplast formation of Rhizoctonia solani in the fast growing anastomosis groups (AGs) 1 and 4, the intermediate AG-2 and AG-5, and the slow AG-3 yielded the most, moderate and the least in that order, respectively. Sclerotla formation varied with AGs. A high yield of protoplasts from AGs was obtained with a combined lytic enzyme system containing cellulase $quot;Onozuka$quot; R-10, macerozyme R-10 and β-glucuronidase. When 3g (fresh weight) of 30 hr old mycelia was incubated for 3 hr at 32℃ with the enzyme mixture in 0.6 M mannitol, maximum protoplasts were obtained in the five AGs. A protoplast fusion between sclerotia forming AG-1 inactivated with heat and non-forming AG-5 was induced by polyethylene glycol and Ca^(2+). Seven fusants obtained were based on characteristics of colony and sclerotium formation on culture plates. The fusants were confirmed by isozyme patterns of esterase and killing reaction between AG-1 and a fusant F1501.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|